Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count.
#Code chicken core 1.0.4 plus
Obtain superior images with the new Invitrogen™ Alexa Fluor™ Plus secondary antibodies. This antibody does not bind non-immunoglobulin chicken serum proteins. Specificity: This antibody binds to heavy chains on chicken IgY and light chains on all chicken immunoglobulins. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use add only the supernatant to the experiment. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.
Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. To minimize cross-reactivity, the goat anti-chicken IgY whole antibodies have been affinity-purified and cross-adsorbed against chicken serum containing non-immunoglobulin chicken serum proteins.
0 kommentar(er)
